Immunochistochemical Characteristic of the Microenvironment in Squamous Cell Lung Cancer

Autor: M. M. Baudarbekova, A. V. Kalmykova
Jazyk: English<br />Russian<br />Ukrainian
Rok vydání: 2017
Předmět:
Zdroj: Patologìâ, Iss 2, Pp 136-141 (2017)
Druh dokumentu: article
ISSN: 2310-1237
2306-8027
DOI: 10.14739/2310-1237.2017.2.109266
Popis: Tumor-associated immunocytes can influence the survival of tumor cells, the processes of proliferation and apoptosis, these immunocytes also participate in the metastatic cascade. Aim. The purpose of this study was to estimate the features of the squamous cell lung cancer (SCLC) microenvironment using the immunohistochemical technique. Materials and methods. Pathomorphological and immunohistochemical studies of the surgical material from 20 patients, which were treated in ZRCOD because of SCLC, were conducted: 10 patients with SCLC without metastases in the regional lymph nodes (RLN) formed the I group and 10 patients with SCLC with metastases in the RLN formed the II group. Results. It was revealed, that the maximums of the distribution density (DD) of CD4+ and CD8+ lymphocytes are typical for the peritumorous sections of the SCLC stroma. Moreover, the statistically significant differences between the CD4+ lymphocytes DD in the stroma of the I and the II groups cases were found: 401.2 (344.6, 511.3) vs. 622.4 (587.2, 793.9) cells/mm2 in the peritumorous zones of the stroma; 298.0 (246.1, 350.1) vs. 484.8 (479.3, 690.6) cells/mm2 in the intertumorous zones of the stroma. The maximum of the CD20+ lymphocytes DD in the I group cases was revealed in the peritumorous zones of the stroma, in the II group cases – in the intertumorous zones of the stroma, while the maximum of the plasmocytes DD was observed in the intertumorous zones of the stroma. The maximum of the macrophages DD is typical for the peritumorous zones of the stroma. Moreover, there are statistically significant differences between the macrophages DD in the intertumorous zones of the stroma in the I and the II groups cases: 259.5 (193.0, 325.2) vs. 507.1 (390.2; 617.8) cells/mm2. Conclusion. SCLC without metastasis in the RLN is characterized by the predominant distribution of CD4+, CD8+ lymphocytes and CD68+ macrophages in the peritumorous zones of the stroma, as well as CD20+ lymphocytes and plasmocytes in the intertumorous zones of the tumor stroma. SCLC with metastases in the RLN differs by the predominance of CD20+ lymphocytes in the peritumorous zones of the stroma, as well as the significantly higher values of CD4+ lymphocytes in the peri- and intertumorous zones of the stroma, CD68+ macrophages in the intertumorous zones of the tumor stroma.
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