Extraction of acid phosphomonoesterase from soil: testing of various extractants

Autor: Ladislav Holík, Peter Dundek, Valerie Vranová, Klement Rejšek, Dalibor Janouš, Pavel Formánek
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Vol 59, Iss 3, Pp 51-54 (2011)
Druh dokumentu: article
ISSN: 20115903
1211-8516
2464-8310
DOI: 10.11118/actaun201159030051
Popis: The aim of this work was to to investigate the suitability of various types of extractants for extraction of acid phosphomonoesterase from soil using various types of extractants. Succinate-borate buffer of pH 4.8 or 0.1 M K2SO4 were the most efficient to extract this enzyme compared to 0.1 M glutamic acid or 0.005 M salicylic acid. Extraction using 0.1 M glutamic acid gave significantly (P
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