Extraction of acid phosphomonoesterase from soil: testing of various extractants
Autor: | Ladislav Holík, Peter Dundek, Valerie Vranová, Klement Rejšek, Dalibor Janouš, Pavel Formánek |
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Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: | |
Zdroj: | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Vol 59, Iss 3, Pp 51-54 (2011) |
Druh dokumentu: | article |
ISSN: | 20115903 1211-8516 2464-8310 |
DOI: | 10.11118/actaun201159030051 |
Popis: | The aim of this work was to to investigate the suitability of various types of extractants for extraction of acid phosphomonoesterase from soil using various types of extractants. Succinate-borate buffer of pH 4.8 or 0.1 M K2SO4 were the most efficient to extract this enzyme compared to 0.1 M glutamic acid or 0.005 M salicylic acid. Extraction using 0.1 M glutamic acid gave significantly (P |
Databáze: | Directory of Open Access Journals |
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