Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response

Autor: Johannes Maximilian Wagner, Felix Reinkemeier, Christoph Wallner, Mehran Dadras, Julika Huber, Sonja Verena Schmidt, Marius Drysch, Stephanie Dittfeld, Henriette Jaurich, Mustafa Becerikli, Kathrin Becker, Nicole Rauch, Vikas Duhan, Marcus Lehnhardt, Björn Behr
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Stem Cells Translational Medicine, Vol 8, Iss 10, Pp 1084-1091 (2019)
Druh dokumentu: article
ISSN: 2157-6580
2157-6564
DOI: 10.1002/sctm.18-0266
Popis: Abstract Bone infections are a frequent cause for large bony defects with a reduced healing capacity. In previous findings, we could already show diminished healing capacity after bone infections, despite the absence of the causing agent, Staphylococcus aureus. Moreover, these bony defects showed reduced osteoblastogenesis and increased osteoclastogenesis, meaning elevated bone resorption ongoing with an elevated B‐cell activity. To overcome the negative effects of this postinfectious inflammatory state, we tried to use the regenerative capacity of mesenchymal stem cells derived from adipose tissue (adipose‐derived stem cells [ASCs]) to improve bone regeneration and moreover were curious about immunomodulation of applicated stem cells in this setting. Therefore, we used our established murine animal model and applicated ASCs locally after sufficient debridement of infected bones. Bone regeneration and resorption as well as immunological markers were investigated via histology, immunohistochemistry, Western blot, and fluorescence‐activated cell scanning (FACS) analysis and μ‐computed tomography (CT) analysis. Interestingly, ASCs were able to restore bone healing via elevation of osteoblastogenesis and downregulation of osteoclasts. Surprisingly, stem cells showed an impact on the innate immune system, downregulating B‐cell population. In summary, these data provide a fascinating new and innovative approach, supporting bone healing after bacterial infections and moreover gain insights into the complex ceremony of stem cell interaction in terms of bone infection and regeneration. Stem Cells Translational Medicine 2019;8:1084–1091
Databáze: Directory of Open Access Journals
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