Modeling gallic acid production rate by empirical and statistical analysis
| Autor: | Bratati Kar, Rintu Banerjee, Bimal Chandra Bhattacharyya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Brazilian Archives of Biology and Technology, Vol 43, Iss 5, Pp 509-513 (2000) |
| Druh dokumentu: | article |
| ISSN: | 1516-8913 1678-4324 |
| DOI: | 10.1590/S1516-89132000000500010 |
| Popis: | For predicting the rate of enzymatic reaction empirical correlation based on the experimental results obtained under various operating conditions have been developed. Models represent both the activation as well as deactivation conditions of enzymatic hydrolysis and the results have been analyzed by analysis of variance (ANOVA). The tannase activity was found maximum at incubation time 5 min, reaction temperature 40ºC, pH 4.0, initial enzyme concentration 0.12 v/v, initial substrate concentration 0.42 mg/ml, ionic strength 0.2 M and under these optimal conditions, the maximum rate of gallic acid production was 33.49 mumoles/ml/min.Para predizer a taxa das reações enzimaticas uma correlação empírica baseada nos resultados experimentais foi desenvolvida. Os modelos representam a ativação e a desativativação da hydrolise enzimatica. Os resultados foram avaliados pela análise de variança (ANOVA). A atividade máxima da tannase foi obtida após 5 minutos de incubação, temperatura 40ºC, pH 4,0, concentração inicial da enzima de 0,12 v/v, concentração inicial do substrato 0,42 mg/ml, força iônica 0,2 M. Sob essas condições a taxa máxima de produção ácido galico foi de 33,49 µmoles/ml/min. |
| Databáze: | Directory of Open Access Journals |
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