| Autor: |
Shin-ichiro Takayanagi, Sayaka Chuganji, Masahiro Tanaka, Bo Wang, Saki Hasegawa, Ken Fukumoto, Nariaki Wasano, Makoto Kakitani, Nakaba Ochiai, Yohei Kawai, Tatsuki Ueda, Akihiro Ishikawa, Yuko Kurimoto, Asami Fukui, Sanae Kamibayashi, Eri Imai, Atsushi Kunisato, Hajime Nozawa, Shin Kaneko |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Communications Biology, Vol 7, Iss 1, Pp 1-14 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2399-3642 |
| DOI: |
10.1038/s42003-024-06297-0 |
| Popis: |
Abstract Memory T cells demonstrate superior in vivo persistence and antitumor efficacy. However, methods for manufacturing less differentiated T cells are not yet well-established. Here, we show that producing chimeric antigen receptor (CAR)-T cells using berbamine (BBM), a natural compound found in the Chinese herbal medicine Berberis amurensis, enhances the antitumor efficacy of CAR-T cells. BBM is identified through cell-based screening of chemical compounds using induced pluripotent stem cell-derived T cells, leading to improved viability with a memory T cell phenotype. Transcriptomics and metabolomics using stem cell memory T cells reveal that BBM broadly enhances lipid metabolism. Furthermore, the addition of BBM downregulates the phosphorylation of p38 mitogen-activated protein kinase and enhanced mitochondrial respiration. CD19-CAR-T cells cultured with BBM also extend the survival of leukaemia mouse models due to their superior in vivo persistence. This technology offers a straightforward approach to enhancing the antitumor efficacy of CAR-T cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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