Soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes): isolated isoforms and kinetics properties
| Autor: | Maria Regina de Aquino-Silva, Maria Luiza Barcellos Schwantes, Flavia Simone Munin, Arno Rudi Schwantes, Silvana Pereira dos Santos |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Genetics and Molecular Biology, Vol 31, Iss 1, Pp 337-342 (2008) |
| Druh dokumentu: | article |
| ISSN: | 1415-4757 1678-4685 |
| DOI: | 10.1590/S1415-47572008000200029 |
| Popis: | Kinetic properties and thermal stabilities of Geophagus brasiliensis skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37) and its isolated isoforms were analyzed to examine a possible sMDH-B* locus duplication in a fixation process influenced by genetic drift. Two optimal pHs were detected: 7.5 for AB1 unfractionated muscle phenotype and its B1 isoform, and 8.0 for AB1B2 unfractionated muscle phenotype, A and B2 isoforms. While G. brasiliensis A isoform could be characterized as thermostable, the duplicated B isoform cannot be assumed as thermolabile. Km values for isolated B2 isoforms were 1.6 times lower than for B1. A duplication event in progress best explains the electrophoretic six-band pattern detected in G. brasiliensis, which would be caused by genetic drift. |
| Databáze: | Directory of Open Access Journals |
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