Antigenic and Genetic Characterization of Twenty-six Strains of Human Respiratory Syncytial Virus (Subgroup A) Isolated During Three Consecutive Outbreaks in Havana City, Cuba
| Autor: | Angel Valdivia, Grehete González, Danay Chacón, Clara Savón, Anselmo Otero, Odalys Váldes, Reynel Cancio, Suset Oropesa, José A Melero, Blanca García-Barreno, Angel Goyenechea |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 94, Iss 4, Pp 469-475 (1999) |
| Druh dokumentu: | article |
| ISSN: | 1678-8060 0074-0276 53800664 |
| DOI: | 10.1590/S0074-02761999000400007 |
| Popis: | Twenty-six human respiratory syncytial virus strains (subgroup A) isolated from three outbreaks in Havana City during the period 1994/95, 1995/96 and 1996/97 were analyzed to determine their antigenic and genetic relationships. Analyses were performed by monoclonal antibodies and restriction mapping (N gene) following amplification of the select region of the virus genome by polymerase chain reaction. All isolated strains were classified as subgroup A by monoclonal antibodies and they showed a restriction pattern NP4 that belonged to subgroup A. Thus the results obtained in this work, showed a close relation (100%) between antigenic and genetic characterization of the isolated strains in our laboratory. These methods permit the examination of large numbers of isolates by molecular techniques, simplifying the researchs into the molecular epidemiology of the virus. |
| Databáze: | Directory of Open Access Journals |
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