A novel prostate cancer immunotherapy using prostate-specific antigen peptides and skin test reagent as an adjuvant
| Autor: | Al-Ola Abdallah, Hannah Coleman, Mohamed Kamel, Rodney Davis, Teri Landrum, Horace Spencer, Sam Mackintosh, Fade A Mahmoud, Natasa Milojkovic, Chester Wicker, Konstantinos Arnaoutakis, Mayumi Nakagawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | SAGE Open Medicine, Vol 6 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2050-3121 20503121 |
| DOI: | 10.1177/2050312118800202 |
| Popis: | Objectives: Our group developed the use of the Candida skin test reagent as an adjuvant of cell-mediated immunity in designing a human papillomavirus therapeutic vaccine. Here, this technology is being applied for designing a prostate cancer immunotherapy. Methods: Peptides based on the prostate-specific antigen amino acid sequences were selected, synthesized, and evaluated in terms of their (1) solubility, (2) maturation effects on Langerhans cells by fluorescence-activated cell sorter analysis, and (3) recognition by peripheral immune cells from prostate cancer patients using interferon-γ enzyme-linked immunospot assay. Results: The peptides were soluble in 10 mM succinate at pH of 5 with 5% glycine, and they demonstrated no maturation effects on Langerhans cells from healthy donors. On the other hand, peripheral immune cells from 4 of 10 prostate cancer patients examined had positive responses in enzyme-linked immunospot assay to one or more prostate-specific antigen peptides. Conclusion: In summary, a design and a formulation of a novel prostate cancer immunotherapy are described. The immunogenicity of prostate-specific antigen peptides in some prostate cancer patients supports further development of this immunotherapy. |
| Databáze: | Directory of Open Access Journals |
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