An Efficient Chemoenzymatic Process for Preparation of Ribavirin
| Autor: | Vladimir Sakharov, Sergey Baykov, Irina Konstantinova, Roman Esipov, Mikhail Dorogov |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | International Journal of Chemical Engineering, Vol 2015 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1687-806X 1687-8078 |
| DOI: | 10.1155/2015/734851 |
| Popis: | Ribavirin is an important antiviral drug, which is used for treatment of many diseases. The pilot-scale chemoenzymatic process for synthesis of the active pharmaceutical ingredient Ribavirin was developed with 32% overall yield and more than 99.5% purity. The described method includes the chemical synthesis of 1,2,4-triazole-3-carboxamide, which is a key intermediate and enzyme-catalyzed transglycosylation reaction for preparation of the desired product. 1,2,4-Triazole-3-carboxamide was synthesized from 5-amino-1,2,4-triazole-3-carboxylic acid by classical Chipen-Grinshtein method. Isolated from E. сoli BL21(DE3)/pERPUPHHO1 strain the purine nucleoside phosphorylase was used as a biocatalytical system. All steps of this process were optimized and scaled. |
| Databáze: | Directory of Open Access Journals |
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