Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro

Autor: Mehdi Shabani, Bahram Nasr Esfahani, Bahar Sadegh Ehdaei, Sharareh Moghim, Arezoo Mirzaei, Mohammadreza Sharifi, Leili Mouhebat
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Research in Pharmaceutical Sciences, Vol 14, Iss 2, Pp 167-174 (2019)
Druh dokumentu: article
ISSN: 1735-5362
1735-9414
DOI: 10.4103/1735-5362.253364
Popis: Herpes simplex virus type 1 (HSV-1) infections are one of the most common diseases in human population. HSV-1 causes subclinical, mild to severe diseases, especially in immunocompromised patients. Acyclovir has been used to reduce manifestations of HSV-1 infections. The extensive use of this drug has led to the development of resistant strains. Thus, designing a novel anti-herpes drug with different mechanisms of action is urgently needed. Cellular microRNAs (miRNAs) have direct antiviral effects in addition to their regulatory functions. In this study we used a novel miRNA (hsa-miR-7704), expressed in macrophages, to inhibit HSV-1 lytic infection in HeLa cells. Synthesized hsa-miR-7704 mimics were transfected into HSV-1 infected HeLa cell. The inhibitory effects of the miRNA were evaluated by plaque assay, real time polymerase chain reaction and the viral titers were measured by the 50% tissue culture infective dose (TCID50). The viral titer and cell cytopathic effect were dramatically decreased in HeLa cells transfected with hsa-miR-7704 (50 and 100 nM), compared with HSV-1 infected cells alone or transfected with the mock miRNA control. These results suggest that hsa-miR-7704 inhibits HSV-1 replication efficiently in vitro. This may provide an alternative mechanism to prevent HSV-1 infections.
Databáze: Directory of Open Access Journals
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