In vitro plant regeneration of lotus (Nelumbo nucifera)
| Autor: | Yu Xia, Sheng Jiajing, Zhao Lingling, Diao Ying, Zheng Xingwen, Xie Keqiang, Zhou Mingquan, Hu Zhongli |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Open Life Sciences, Vol 10, Iss 1 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2391-5412 2015-0016 |
| DOI: | 10.1515/biol-2015-0016 |
| Popis: | In this study, an efficient and reproducible plant regeneration system for lotus (Nelumbo nucifera Gaertn.) was established using shoot apical meristems from the buds and one-week-old aseptically germinated embryos as explants. Multiple shoot clumps were induced on Murashige and Skoog (MS) basal medium supplemented with various combinations of N6-Benzylaminopurine (6-BA) and α-Naphthalene acetic acid (NAA). The maximum response was obtained with 2.22 μM 6-BA, and produced 21.33 shoots per explant after four weeks. After five subcultures, multiple shoot clumps were transferred to MS basal medium supplemented with various combinations of 3-Indolebutyric acid (IBA), NAA and sucrose for root induction. After four weeks, plantlets with well-developed roots were achieved on MS basal medium supplemented with 0.54 μM NAA and 30 g/L sucrose with 100% rooting rate. The successfully acclimated plantlets were transferred to pots with the addition of 2 g/L KMnO4 into the soils, and finally fertile plants with much bigger leaves were obtained in the greenhouse. The survival rate was 97.33%. |
| Databáze: | Directory of Open Access Journals |
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