| Autor: |
Yeh-Sheng Chien, Shu-Ting Luo, Kuo-Chien Tsao, Yhu-Chering Huang, Wan-Yu Chung, Yu-Chieh Liao, Yi Tan, Suman R. Das, Min-Shi Lee |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Journal of Biomedical Science, Vol 26, Iss 1, Pp 1-11 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1423-0127 |
| DOI: |
10.1186/s12929-019-0541-x |
| Popis: |
Abstract Background Human enteroviruses contain over 100 serotypes. We have routinely conducted enterovirus surveillance in northern Taiwan; but about 10% of isolates could not be serotyped using traditional assays. Next-generation sequencing (NGS) is a powerful tool for genome sequencing. Methods In this study, we established an NGS platform to conduct genome sequencing for the serologically untypable enterovirus isolates. Results Among 130 serologically untypable isolates, 121 (93%) of them were classified into 29 serotypes using CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer)-based RT-PCR to amplify VP1 genes (VP1-CODEHOP). We further selected 52 samples for NGS and identified 59 genome sequences from 51 samples, including 8 samples containing two virus genomes. We also detected 23 genome variants (nucleotide identity |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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