Immunohistochemical detection of p53 and pp53 Ser392 in canine hemangiomas and hemangiosarcomas located in the skin

Autor: María José García-Iglesias, Jose Luis Cuevas-Higuera, Ana Bastida-Sáenz, María Gracia de Garnica-García, Laura Polledo, Paula Perero, Jorge González-Fernández, Beatriz Fernández-Martínez, Claudia Pérez-Martínez
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: BMC Veterinary Research, Vol 16, Iss 1, Pp 1-13 (2020)
Druh dokumentu: article
ISSN: 1746-6148
DOI: 10.1186/s12917-020-02457-6
Popis: Abstract Background p53 protein is essential for the regulation of cell proliferation. Aberrant accumulation of it usually occurs in cutaneous malignancies. Mutant p53 is detected by immunohistochemistry because it is more stable than the wild-type p53. However, post-translational modifications of p53 in response to ultraviolet radiation are important mechanisms of wild-type p53 stabilization, leading to positive staining in the absence of mutation. The aims were: 1) to analyze the immunohistochemical expression of p53 and phospho-p53 Serine392 in canine skin endothelial tumours; and 2) to determine if any relationship exists between p53 and phospho-p53 Serine392 overexpression and cell proliferation. Results p53 and phospho-p53 Serine392 immunolabeling was examined in 40 canine cutaneous endothelial tumours (13 hemangiomas and 27 hemangiosarcomas). Their expression was associated with tumour size, hemangiosarcoma stage (dermal versus hypodermal), histological diagnosis and proliferative activity (mitotic count and Ki-67 index). Statistical analysis revealed a significant increase of p53 immunoreactivity in hemangiosarcomas (median, 74.61%; interquartile range [IQR], 66.97–82.98%) versus hemangiomas (median, 0%; IQR, 0–20.91%) (p
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