Indole Derivative Feeding Test and Detection of TRP and Indole derivatives by Thin Layer Chromatography

Autor: Magdalena Hilbert, Lars Voll, Jörg Hofmann, Alga Zuccaro
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Bio-Protocol, Vol 3, Iss 12 (2013)
Druh dokumentu: article
ISSN: 2331-8325
DOI: 10.21769/BioProtoc.801
Popis: The mutualistic root endophyte Piriformospora indica colonizes a wide range of plants and the colonization of root cells by this fungus is very often associated with beneficial effects to its host, such as growth promotion and increased biotic and abiotic stress tolerance. These traits could be based on general mechanisms and signaling pathways common to many different plant species. One such mechanism could be the recruitment of phytohormone pathways by P. indica. It is known, that many mutualistic microorganisms are able to synthesize and secrete phytohormones during the interaction with their host plants. This protocol has been successfully utilized to analyze tryptophan (TRP)-dependent biosynthesis of indole-3-acetic acid (IAA) and its indole derivatives by P. indica (Hilbert et al., 2012).
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