Alpha-, gamma- and beta-proteobacteria detected in legume nodules in Latvia, using full-length 16S rRNA gene sequencing

Autor: A. Klūga, L. Dubova, I. Alsiņa, N. Rostoks
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Acta Agriculturae Scandinavica. Section B, Soil and Plant Science, Vol 73, Iss 1, Pp 127-141 (2023)
Druh dokumentu: article
ISSN: 0906-4710
1651-1913
09064710
DOI: 10.1080/09064710.2023.2232681
Popis: Rhizobia are soil bacteria that form nodules on legumes and fix atmospheric nitrogen, reducing the need for chemical fertilisers. In most studies rhizobia are described as alpha-proteobacteria and have been studied extensively for several decades. However, in recent years an increasing number of studies present beta- and gamma-proteobacteria strains as potential rhizobia-like bacteria. In this study we identify bacteria isolated from nodules of various legumes grown in Latvia, using a full-length 16S rRNA gene sequence, used for more precision, compared with a partial sequence of the gene. Bacterial strains in this study have been isolated from 1962–2019, part of which are rhizobia strains from a unique historical collection. This is the first time bacterial strains isolated from nodules in Latvian soils have been identified with molecular biology methods. A wide taxonomic diversity was detected – bacteria species from α-, β-, γ-proteobacteria classes and Paenibacillus polymyxa strains from the Bacilli class. P. polymyxa strains were detected only in the historical collection, while β- and γ-proteobacteria strains were obtained only from the newly isolated specimens, uncovering new potential strains for a commercial legume seed inoculum. Soil samples were collected, and phosphorus levels were determined. Several strains indicate phosphate solubilising properties.
Databáze: Directory of Open Access Journals
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