Aneuploidy and improved growth are coincident but not causal in a yeast cancer model.
| Autor: | Xin Chenglin Li, John C Schimenti, Bik K Tye |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | PLoS Biology, Vol 7, Iss 7, p e1000161 (2009) |
| Druh dokumentu: | article |
| ISSN: | 1544-9173 1545-7885 43114458 |
| DOI: | 10.1371/journal.pbio.1000161 |
| Popis: | Cancer cells have acquired mutations that alter their growth. Aneuploidy that typify cancer cells are often assumed to contribute to the abnormal growth characteristics. Here we test the idea of a link between aneuploidy and mutations allowing improved growth, using Saccharomyces cerevisiae containing a mcm4 helicase allele that was shown to cause cancer in mice. Yeast bearing this mcm4 allele are prone to undergoing a "hypermutable phase" characterized by a changing karyotype, ultimately yielding progeny with improved growth properties. When such progeny are returned to a normal karyotype by mating, their improved growth remains. Genetic analysis shows their improved growth is due to mutations in just a few loci. In sum, the effects of the mcm4 allele in mice are recapitulated in yeast, and the aneuploidy is not required to maintain improved growth. |
| Databáze: | Directory of Open Access Journals |
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