| Autor: |
Yanbin ZHU, Xinting XU, Yanbing LI, Chengming ZHAO, Qingnian HUANG, Zhibao CHEN |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Shipin gongye ke-ji, Vol 44, Iss 19, Pp 401-409 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1002-0306 |
| DOI: |
10.13386/j.issn1002-0306.2022100017 |
| Popis: |
To explore the effect of Inotolinol (INO) on the inflammatory response of LPS-induced mouse mononuclear macrophages (RAW264.7). The experiment has investigated in vitro the effects of INO on mouse mononuclear macrophages (RAW264.7) induced by LPS. To do this, the following methods were adopted: The viability of RAW264.7 cells was detected by CCK-8 assay. Cell apoptosis was examined by Hoechst33342 and PI. The secretion of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-18 (IL-18) and the content of nitric oxide (NO) in cells were measured by ELISA and Griess. The production of reactive oxygen species (ROS), the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) in cells were detected by fluorescence probe and kit. The results showed that 20 μmol/L INO could inhibit the decline of RAW264.7 cell viability induced by LPS, prevent cell apoptosis, and significantly inhibit the secretion of IL-6, IL-1β, TNF-α, IL-18 and NO (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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