Protein fraction heterogeneity in donkey’s milk analysed by proteomic methods

Autor: G. D'Urso, A. M. Guastella, S. Bordonaro, V. Cunsolo, A. Zuccaro, V. Muccilli, A. Criscione, D. Marletta
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Italian Journal of Animal Science, Vol 6, Iss 1s, Pp 650-652 (2010)
Druh dokumentu: article
ISSN: 1594-4077
1828-051X
DOI: 10.4081/ijas.2007.1s.650
Popis: Donkey’s milk is often well tolerate by patients affected by cow’s milk protein allergy, probably thanks to its protein composition. This empiric evidence, confirmed by some clinical trials, needs to be better investigated. A preliminary survey on the protein fraction of donkey’s milk was carried out: fifty-six individual milk samples have been collected and analysed by IEF and SDS-PAGE. Five different IEF patterns have been identified, showing a marked heterogeneity both in casein and whey protein fractions. A single IEF pattern showed an apparent reduced amount of casein fraction highlighted by SDS. Three of the five IEF patterns have been further investigated by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS).
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