Evaluation of the FAS and FASL Gene changes in women with premature ovarian failure: A case-control study
| Autor: | Zhima Akhavansales, Alimohammad Mosadeghmehrjardi, Hamid Reza Ashrafzadeh, Shadnaz Fakhteh Yavari, Mohammad Taher Tahoori, Morteza Bitaraf Sani, Mahnaz Mohammadi, Fateme Montazeri, Nasrin Ghasemi |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International Journal of Reproductive BioMedicine, Vol 20, Iss 12, Pp 1007-1012 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2476-4108 2476-3772 |
| DOI: | 10.18502/ijrm.v20i12.12561 |
| Popis: | Abstract Background: Premature ovarian failure (POF), is menopause occurring before the age of 40, affecting 1-3% of women worldwide. The risk of POF increases with altered immunological parameters such as FAS and FASL genes, which play a fundamental role in embryogenesis and cellular homeostasis. Objective: The study aimed to investigate the potential role of FAS and FASL genes in POF pathogenesis. Materials and Methods: In this case-control study, the polymorphisms of FAS-670A/G and FASLIVS2nt_124A/G apoptotic genes were analyzed in 51 Iranian women suffering from POF, and 61 healthy controls. Isolation of DNA was done using the salting-out method, and genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method. Results: Our results revealed that homozygous FAS-670A/A and G/G, and heterozygous FAS-670A/G are not significantly different between cases and controls (p = 0.99). Also, in different genotyping models of FASIVS2nt_124, polymorphisms were not related to POF risk (p = 0.23). Conclusion: There is no statistical association between these polymorphisms and POF risk in women referred to genetic counseling clinics. |
| Databáze: | Directory of Open Access Journals |
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