| Autor: |
Pavlović Radmila, Biondi Antonio Pier, Chiesa Maria Luca, Trutić Nataša, Abramović Mirjana, Santaniello Enzo |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Journal of the Serbian Chemical Society, Vol 77, Iss 5, Pp 667-683 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
0352-5139 |
| DOI: |
10.2298/JSC110304003P |
| Popis: |
In view to develop a gas-chromatographic (GC) determination of the 3-nitrotyrosine (NY)/tyrosine (Y) ratio as a marker of nitro-oxidative stress, different reagents were tested with the objective of obtaining a single volatile fluorinated product for each amino acid by a one-step derivatization procedure. The heptafluorobutyric anhydride (HFBA) /heptafluorobutanol (HFBOH) mixture proved unsuccessful for NY and Y simultaneous analysis. The reaction with different chloroformates [isobutyl chlorofomate (iBuCF) and ethyl chloroformate (EtCF)] in the presence of different perfluorinated alcohols such as trifluoroethanol (TFEOH) and HFBOH was investigated. Combination EtCF/fluorinated alcohols yielded derivatives of NY and Y as single peaks suitable to the GC determination of the NY/Y ratio. The different behaviour of two amino acids in the used reaction mixtures and the parameters influencing the results were discussed. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
