Investigation of the Properties of Immobilized Horseradish Peroxidase on Magnetic Particles
| Autor: | Ivanov T., Yotova L., Mironova V., Chuchuranova V. |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Bioautomation, Vol 11, Iss 1, Pp 65-72 (2008) |
| Druh dokumentu: | article |
| ISSN: | 1313-261X 1312-451X |
| Popis: | Magnetic particles coated with copolymer of acrylamide and acrylonitrile have been prepared. Those particles were used as a matrix for a covalent binding of peroxidase. The periodic-oxidized enzyme was bound to the matrix by immobilization procedure at 4°C for 18 hours. The immobilized enzyme showed relative activity of 86%. The following results were obtained for pH and optimum temperature of the immobilized enzyme - 7.0 and 30°C, respectively. The analysis of the kinetic parameters of the immobilized enzyme showed values of Vmax - 0.0517 M.10-6/sec and Km - 2.3x10-4 M. |
| Databáze: | Directory of Open Access Journals |
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