Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation

Autor: TATJANA ŠOLEVIĆ, MILAN NOVAKOVIĆ, MILA ILIĆ, MALIŠA ANTIĆ, MIROSLAV M. VRVIĆ, BRANIMIR JOVANČIĆEVIĆ
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Journal of the Serbian Chemical Society, Vol 76, Iss 3, Pp 425-438 (2011)
Druh dokumentu: article
ISSN: 0352-5139
Popis: The bioremediation potential of the aerobic zymogenous microorganisms in soil (Danube alluvium, Pančevo, Serbia) for crude oil biodegradation was investigated. A mixture of paraffinic types of oils was used as the substrate. The laboratory experiment of the simulated oil biodegradation lasted 15, 30, 45, 60 and 75 days. In parallel, an experiment with a control sample was conducted. Extracts were isolated from the samples with chloroform in a separation funnel. From these extracts, the hydrocarbons were isolated by column chromatography and analyzed by gas chromatography–mass spectrometry (GC–MS). n-Alkanes, isoprenoids, phenanthrene and its derivatives with one and two methyl groups were quantitatively analyzed. The ability and efficiency of zymogenous microorganisms in soil for crude oil bioremediation was assessed by comparison between the composition of samples which were exposed to the microorganisms and the control sample. The investigated microorganisms showed the highest bioremediation potential in the biodegradation of n-alkanes and isoprenoids. A considerably high bioremediation potential was confirmed in the biodegradation of phenanthrene and methyl phenanthrenes. Low bioremediation potential of these microorganisms was proven in the case of polycyclic alkanes of the sterane and triterpane types and dimethyl phenanthrenes.
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