| Autor: |
M.F. HOLICK, H.F. DeLUCA |
| Jazyk: |
angličtina |
| Rok vydání: |
1971 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 12, Iss 4, Pp 460-465 (1971) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)39495-5 |
| Popis: |
A simple yet powerful new chromatographic procedure for vitamin D3 and its metabolites is described. Liquid–gel partition chromatography on Sephadex LH-20 using a solvent of various percentages of CHCl3 in Skellysolve B (petroleum ether, bp 67–69°C) permits excellent resolution of vitamin D3, 25-hydroxyvitamin D3, and their more polar metabolites. Of special importance is the resolution of the metabolites of vitamin D3 more polar than 25-hydroxycholecalciferol. Because of this resolution, a new metabolite of vitamin D3 has been demonstrated in the plasma of rats and in the intestines of chicks given 100 IU of vitamin D3-1,2-3H. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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