Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

Autor: Edward King, Sarah Maxel, Yulai Zhang, Karissa C. Kenney, Youtian Cui, Emma Luu, Justin B. Siegel, Gregory A. Weiss, Ray Luo, Han Li
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-022-35021-x
Popis: Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH).
Databáze: Directory of Open Access Journals