Isolation of High-Molecular-Length DNA from Human Skin
Autor: | P.V. Bennett, R.W. Gange, H. Hacham, V.S. Hejmadi, M. Moran, S. Ray, B.M. Sutherland |
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Jazyk: | angličtina |
Rok vydání: | 1996 |
Předmět: | |
Zdroj: | BioTechniques, Vol 21, Iss 3, Pp 458-463 (1996) |
Druh dokumentu: | article |
ISSN: | 1940-9818 0736-6205 |
DOI: | 10.2144/96213st03 |
Popis: | An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies. |
Databáze: | Directory of Open Access Journals |
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