Isolation of High-Molecular-Length DNA from Human Skin

Autor: P.V. Bennett, R.W. Gange, H. Hacham, V.S. Hejmadi, M. Moran, S. Ray, B.M. Sutherland
Jazyk: angličtina
Rok vydání: 1996
Předmět:
Zdroj: BioTechniques, Vol 21, Iss 3, Pp 458-463 (1996)
Druh dokumentu: article
ISSN: 1940-9818
0736-6205
DOI: 10.2144/96213st03
Popis: An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies.
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