| Autor: |
Jiao WU, Ya-li ZHANG, Rong-rong HE, Lei ZHU, Chao-xia WANG, Xia XU, Jiang LU |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Journal of Integrative Agriculture, Vol 11, Iss 3, Pp 387-396 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
2095-3119 |
| DOI: |
10.1016/S2095-3119(12)60023-9 |
| Popis: |
Vitis vinifera cv. Chardonnay suspension cultures were established from proembryogenic mass and employed for optimizing Agrobacterium-mediated transformation system. One-factor-at-a-time experiment revealed that OD600 of Agrobacterium, time of inoculation, co-cultivation, and cell-drying before inoculation significantly affected the transformation efficiency which reached maximum 21.5% at the following conditions: 0.8 of OD600, 25 min of inoculation, 2 d of co-cultivation, and 10 min of cell drying. Response surface methodology experiments based on a five-level, four-factor central-composite rotatable design were then used to optimize these selected factors. The optimized conditions for Chardonnay grape transformation were: 0.8711 of OD600, 28.9 min of inoculation, 2.25 d of co-cultivation and 11.76 min of cell drying. After optimization, transformation efficiency was 26.2% and there were no interactions among different factors. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect