| Autor: |
Yang Lv, Chen-Wei Gao, Bo Liu, Hao-Yu Wang, Hai-Ping Wang |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Kaohsiung Journal of Medical Sciences, Vol 33, Iss 10, Pp 477-485 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1607-551X |
| DOI: |
10.1016/j.kjms.2017.06.006 |
| Popis: |
The present study tested the hypotheses that bone morphogenetic protein 2(BMP-2) combined with salvianolic acid B(Sal-B) enhance the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) towards cardiomyocytes in vitro. BMSCs were treated with BMP-2 and Sal-B, alone or in combination, for 72 h, then added new media (excluding inductive substance) and cultured for 4 weeks. The morphologic characteristics, surface antigens and mRNA expression of several transcription factors were also assessed. We found that they could all be identified by the positive staining for cardiomyocyte-specific proteins, desmin and cardiac troponin T, in these cells. Furthermore, the mRNA expression of GATA-4 and Nkx2.5 genes was slightly increased on day 7, enhanced on day 14 and decreased on day 28 while α-MHC gene was not expressed on day 7, but expressed slightly on day 14 and enhanced on day 28. The expression of these cardiac-specific markers in treatment groups were all significantly higher than those in the control group, respectively (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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