Copper Staining/Labeling and Scanning Electrochemical Microscopy Readout of Proteins on Poly(vinylidene difluoride) Membranes
| Autor: | Maurizio Carano, Niels Lion, Hubert H. Girault |
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| Jazyk: | German<br />English<br />French |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | CHIMIA, Vol 59, Iss 3 (2005) |
| Druh dokumentu: | article |
| ISSN: | 0009-4293 2673-2424 |
| DOI: | 10.2533/000942905777676849 |
| Popis: | Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving copper salts. The latter are then reduced to copper metal and further detected by SECM with ferrocene methanol as a redox mediator in aqueous solution. During the SECM scan, the potential is held at a value at which the oxidation of the redox mediator occurs and a positive feedback current is detected when scanning over copper clusters. A negative feedback is observed elsewhere. |
| Databáze: | Directory of Open Access Journals |
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