| Autor: |
Zachary William Cutts, Jessica M. Hong, Shirley Shao, Alexander Tran, Michelle Dimon, Marc Berndl, Diana Wu, Annalisa Pawlosky |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 4, Pp 101724- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101724 |
| Popis: |
Summary: Systematic evolution of ligands by exponential enrichment (SELEX) encompasses a wide variety of high-throughput screening techniques for producing nucleic acid binders to molecular targets through directed evolution. We describe here the design and selection steps for discovery of DNA aptamers with specificity for the two consecutive N-terminal amino acids (AAs) of a small peptide (8–10 amino acids). This bead-based method may be adapted for applications requiring binders which recognize a specific portion of the desired target.For complete details on the use and execution of this protocol, please refer to Hong et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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