Rapid microfluidic isolation of virally infected primary bronchial epithelial cells for single-cell RNA sequencing

Autor:	Lucy M Kimbley, Rachel Parker, Maaike Sybil Jongen, John W Holloway, Emily J Swindle, Matthew JJ Rose-Zerilli
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	bronchial epithelium microfluidic device scRNA-seq single cell isolation virus Biology (General) QH301-705.5
Zdroj:	BioTechniques, Vol 71, Iss 1, Pp 387-391 (2021)
Druh dokumentu:	article
ISSN:	1940-9818 0736-6205
DOI:	10.2144/btn-2021-0020
Popis:	Single-cell RNA sequencing (scRNA-seq) of the bronchial epithelium enables examination of cellular subtypes and their responses to viral infections. Here, an optimized method for the isolation of virally infected primary bronchial epithelial cells using a commercially available microfluidic device is presented. Using this method single cells can be rapidly isolated with minimal equipment available in most laboratories. Isolation can be carried out inside biological safety cabinets, permitting the use of virally infected cells. Both cell-line and primary cells isolated using the device retained sufficient RNA integrity for the generation of short-read sequencing-compatible cDNA libraries to facilitate scRNA-seq.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/414344b6eb0145d0816cdde2bd71dad1 Zobrazit plný text záznamu View record in DOAJ