Multi-Cohort Transcriptomic Profiling of Medical Gas Plasma-Treated Cancers Reveals the Role of Immunogenic Cell Death

Autor: Antonios Gkantaras, Charalampos Kotzamanidis, Konstantinos Kyriakidis, Evangelia Farmaki, Kali Makedou, Georgios Tzimagiorgis, Sander Bekeschus, Andigoni Malousi
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Cancers, Vol 16, Iss 12, p 2186 (2024)
Druh dokumentu: article
ISSN: 16122186
2072-6694
DOI: 10.3390/cancers16122186
Popis: The therapeutic potential of cold physical gas plasma operated at atmospheric pressure in oncology has been thoroughly demonstrated in numerous preclinical studies. The cytotoxic effect on malignant cells has been attributed mainly to biologically active plasma-generated compounds, namely, reactive oxygen and nitrogen species. The intracellular accumulation of reactive oxygen and nitrogen species interferes strongly with the antioxidant defense system of malignant cells, activating multiple signaling cascades and inevitably leading to oxidative stress-induced cell death. This study aims to determine whether plasma-induced cancer cell death operates through a universal molecular mechanism that is independent of the cancer cell type. Using whole transcriptome data, we sought to investigate the activation mechanism of plasma-treated samples in patient-derived prostate cell cultures, melanoma, breast, lymphoma, and lung cancer cells. The results from the standardized single-cohort gene expression analysis and parallel multi-cohort meta-analysis strongly indicate that plasma treatment globally induces cancer cell death through immune-mediated mechanisms, such as interleukin signaling, Toll-like receptor cascades, and MyD88 activation leading to pro-inflammatory cytokine release and tumor antigen presentation.
Databáze: Directory of Open Access Journals
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