Glycated ferritin increases the in vitro expression of TLR2 and TLR4 in peripheral blood CD14+ cells obtained from patients with prediabetes
| Autor: | Galván-Moroyoqui José Manuel, Martínez-Soto Juan Manuel, López-Soto Luis Fernando, Soto-Guzmán Jesús Adriana, Camacho-Villa Alma Yolanda, Alvarez-Hernandez Gerardo, Mata-Pineda Ana Lourdes, Candia-Plata Maria Del Carmen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Archives of Biological Sciences, Vol 72, Iss 3, Pp 305-312 (2020) |
| Druh dokumentu: | article |
| ISSN: | 0354-4664 1821-4339 20032102 |
| DOI: | 10.2298/ABS200321024G |
| Popis: | Serum ferritin is a widely-used marker of inflammation in prediabetes, diabetes and atherosclerosis. In these cases, progressive endothelial damage may involve the participation of toll-like receptors (TLR). The aim of this study was to determine the expression of TLR2 and TLR4 in peripheral blood mononuclear cell (PBMC)-derived CD14+ cells from subjects with prediabetes and with a high level of serum ferritin both at baseline and after in vitro cell stimulation with glycated ferritin. Blood samples were drawn from 22 subjects (13 with prediabetes and 9 with normoglycemia). Serum ferritin levels were measured by ELISA, while the expression of TLR2 and TLR4 in PBMC-derived CD14+ cells was determined by flow cytometry. IL-6 and IL-8 cytokines in PBMC-derived CD14+ supernatants were measured by ELISA. Subjects with prediabetes had a higher baseline expression of TLR4 in PBMC-derived CD14+ cells than was observed in cells from normoglycemic subjects (p |
| Databáze: | Directory of Open Access Journals |
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