Biosynthesis, characterization and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus

Autor: Tej Singh, Kumari Jyoti, Amar Patnaik, Ajeet Singh, Ranchan Chauhan, S.S. Chandel
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Journal of Genetic Engineering and Biotechnology, Vol 15, Iss 1, Pp 31-39 (2017)
Druh dokumentu: article
ISSN: 1687-157X
DOI: 10.1016/j.jgeb.2017.04.005
Popis: In this study, biological synthesis of silver nanoparticles (AgNPs) from supernatant of endophytic fungus Alternaria sp. isolated from the healthy leaves of Raphanus sativus is studied. The synthesized AgNPs are characterized using UV-vis spectroscopy and Fourier transform-infrared spectroscopy (FTIR). The structural analysis is done by powder X-ray diffraction (XRD) method. The stability of AgNPs is studied by dynamic light scattering (DLS) method. The size and shape of AgNPs are observed by transmission electron microscopy (TEM) and atomic force microscopy (AFM) and found to be spherical with an average particles size of 4–30 nm. Further, these AgNPs have been found to be highly toxic against human pathogenic bacteria, suggesting the possibility of using AgNPs as efficient antibacterial agents.
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