| Autor: |
Lukas B. Moser, Christoph Bauer, Alexander Otahal, Daniela Kern, Dietmar Dammerer, Thore Zantop, Stefan Nehrer |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of Experimental Orthopaedics, Vol 10, Iss 1, Pp n/a-n/a (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2197-1153 |
| DOI: |
10.1186/s40634-023-00661-5 |
| Popis: |
Abstract Purpose The study aimed to compare the effect of mincing bovine articular cartilage with different shaver blades on chondrocyte viability. Methods Bovine articular cartilage was harvested either with a scalpel or with three different shaver blades (2.5 mm, 3.5 mm, or 4.2 mm) from a commercially available shaver. The cartilage harvested with a scalpel was then minced into fragments smaller than 1 mm3 with a scalpel. All four conditions were cultivated in a culture medium for seven days. After Day 1 and Day 7, the following measurements were performed: metabolic activity, RNA isolation, and gene expression of anabolic (COL2A1 and ACAN) and catabolic genes (MMP1 and MMP13), live/dead staining and visualization using confocal microscopy, and flow cytometric characterization of minced cartilage chondrocytes. Results Mincing the cartilage with shavers significantly reduced metabolic activity after one and seven days compared to scalpel mincing (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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