Autor: |
Hafez Al-Momani, Iman Aolymat, Lujain Ibrahim, Hadeel Albalawi, Dua’a Al Balawi, Borhan Aldeen Albiss, Muna Almasri, Sahar Alghweiri |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
|
Zdroj: |
BMC Microbiology, Vol 24, Iss 1, Pp 1-17 (2024) |
Druh dokumentu: |
article |
ISSN: |
1471-2180 |
DOI: |
10.1186/s12866-024-03441-y |
Popis: |
Abstract Introduction Hormesis describes an inverse dose-response relationship, whereby a high dose of a toxic compound is inhibitory, and a low dose is stimulatory. This study explores the hormetic response of low concentrations of zinc oxide nanoparticles (ZnO NPs) toward Pseudomonas aeruginosa. Method Samples of P. aeruginosa, i.e. the reference strain, ATCC 27,853, together with six strains recovered from patients with cystic fibrosis, were exposed to ten decreasing ZnO NPs doses (0.78–400 µg/mL). The ZnO NPs were manufactured from Peganum harmala using a chemical green synthesis approach, and their properties were verified utilizing X-ray diffraction and scanning electron microscopy. A microtiter plate technique was employed to investigate the impact of ZnO NPs on the growth, biofilm formation and metabolic activity of P. aeruginosa. Real-time polymerase chain reactions were performed to determine the effect of ZnO NPs on the expression of seven biofilm-encoding genes. Result The ZnO NPs demonstrated concentration-dependent bactericidal and antibiofilm efficiency at concentrations of 100–400 µg/mL. However, growth was significantly stimulated at ZnO NPs concentration of 25 µg/mL (ATCC 27853, Pa 3 and Pa 4) and at 12.5 µg/mL and 6.25 µg/mL (ATCC 27853, Pa 2, Pa 4 and Pa 5). No significant positive growth was detected at dilutions |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
|