| Autor: |
Paolo Fattorini, Giorgio Marrubini, Serena Bonin, Barbara Bertoglio, Pierangela Grignani, Elisa Recchia, Paola Pitacco, Francesca Procopio, Carolina Cantoni, Irena Zupanič Pajnič, Solange Sorçaburu-Cigliero, Carlo Previdere` |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Data in Brief, Vol 20, Iss , Pp 1237-1243 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2352-3409 |
| DOI: |
10.1016/j.dib.2018.08.120 |
| Popis: |
This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0–36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0–10 h in a previous study. Keywords: qPCR, Degraded samples, DNA quantification, DNA hydrolysis |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
