Role of platelet count in a murine stasis model of deep vein thrombosis
| Autor: | Rick Mathews, Naly Setthavongsack, Anh Le-Cook, Andy Kaempf, Jennifer M Loftis, Randall L Woltjer, Christina U Lorentz, Alexey Revenko, Monica T Hinds, Khanh P Nguyen |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Platelets, Vol 35, Iss 1 (2024) |
| Druh dokumentu: | article |
| ISSN: | 09537104 1369-1635 0953-7104 |
| DOI: | 10.1080/09537104.2023.2290916 |
| Popis: | AbstractPlatelets are core components of thrombi but their effect on thrombus burden during deep vein thrombosis (DVT) has not been fully characterized. We examined the role of thrombopoietin-altered platelet count on thrombus burden in a murine stasis model of DVT. To modulate platelet count compared to baseline, CD1 mice were pretreated with thrombopoietin antisense oligonucleotide (THPO-ASO, 56% decrease), thrombopoietin mimetic (TPO-mimetic, 36% increase), or saline (within 1%). Thrombi and vein walls were examined on postoperative days (POD) 3 and 7. Thrombus weights on POD 3 were not different between treatment groups (p = .84). The mean thrombus weights on POD 7 were significantly increased in the TPO-mimetic cohort compared to the THPO-ASO (p = .005) and the saline (p = .012) cohorts. Histological grading at POD 3 revealed a significantly increased smooth muscle cell presence in the thrombi and CD31 positive channeling in the vein wall of the TPO-mimetic cohort compared to the saline and THPO-ASO cohorts (p |
| Databáze: | Directory of Open Access Journals |
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