| Autor: |
Hideaki Nishihara, Benjamin D. Gastfriend, Pelin Kasap, Sean P. Palecek, Eric V. Shusta, Britta Engelhardt |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 2, Iss 2, Pp 100563- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100563 |
| Popis: |
Summary: We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion.For complete details on the use and execution of this protocol, please refer to Lian et al. (2014), Nishihara et al. (2020a). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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