Shiga Toxin-Producing Escherichia coli Strains from Romania: A Whole Genome-Based Description
| Autor: | Codruța-Romanița Usein, Mihaela Oprea, Sorin Dinu, Laura-Ioana Popa, Daniela Cristea, Cornelia-Mădălina Militaru, Andreea Ghiță, Mariana Costin, Ionela-Loredana Popa, Anca Croitoru, Cristina Bologa, Lavinia-Cipriana Rusu |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Microorganisms, Vol 12, Iss 7, p 1469 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2076-2607 84019948 |
| DOI: | 10.3390/microorganisms12071469 |
| Popis: | The zoonotic Shiga toxin-producing Escherichia coli (STEC) group is unanimously regarded as exceptionally hazardous for humans. This study aimed to provide a genomic perspective on the STEC recovered sporadically from humans and have a foundation of internationally comparable data. Fifty clinical STEC isolates, representing the culture-confirmed infections reported by the STEC Reference Laboratory between 2016 and 2023, were subjected to whole-genome sequencing (WGS) analysis and sequences were interpreted using both commercial and public free bioinformatics tools. The WGS analysis revealed a genetically diverse population of STEC dominated by non-O157 serogroups commonly reported in human STEC infections in the European Union. The O26:H11 strains of ST21 lineage played a major role in the clinical disease resulting in hospitalisation and cases of paediatric HUS in Romania surpassing the O157:H7 strains. The latter were all clade 7 and mostly ST1804. Notably, among the Romanian isolates was a stx2a-harbouring cryptic clade I strain associated with a HUS case, stx2f- and stx2e-positive strains, and hybrid strains displaying a mixture of intestinal and extraintestinal virulence genes were found. As a clearer picture emerges of the STEC strains responsible for infections in Romania, further surveillance efforts are needed to uncover their prevalence, sources, and reservoirs. |
| Databáze: | Directory of Open Access Journals |
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