Production of Extracellular Lipase from Aspergillus niger by Solid-State Fermentation
| Autor: | Janny Coca Armas, Julio C. Dustet Mendoza, José L. Martínez Hernández, Gwen Falony |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Food Technology and Biotechnology, Vol 44, Iss 2, Pp 235-240 (2006) |
| Druh dokumentu: | article |
| ISSN: | 1330-9862 1334-2606 |
| Popis: | Lipase production in Aspergillus niger J-1 was tested using both submerged fermentation (SmF) and solid-state fermentation (SSF) on a mineral culture medium and wheat bran, respectively. The optimization of the culture medium was carried out for both SmF and SSF. The maximum lipase activity, 1.46 IU/mL, was obtained during the submerged fermentation in a medium containing glucose at 2 % and olive oil at 2 % under conditions of 1 vvm and 450 m–1. However, 9.14 IU/g of dry solid substrate equivalent to 4.8 IU/mL of lipase activity was reached using solid-state fermentation process with a medium containing 0.75 % of ammonium sulphate and 0.34 % of urea. The optimum pH and temperature for enzymatic activity were pH=6 and 40 °C, respectively. The enzyme also exhibited 80 % of its initial activity in neutral and mildly acid media and at temperatures between 20 and 30 °C for a period of 24 hours. |
| Databáze: | Directory of Open Access Journals |
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