Distribution of Glycoconjugates terminal sugars during neurohypophysis development in Rat
| Autor: | Ebrahimzadeh Bideskan AR (PhD), Nikravesh MR (PhD), Fazel AR (PhD) |
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| Jazyk: | perština |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | مجله دانشگاه علوم پزشکی گرگان, Vol 13, Iss 3, Pp 26-34 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1562-4765 2008-4080 |
| Popis: | Background and Objective: The neurohypophysis originates from the floor of diencephalon. Its development controles by several cellular interactions that mediated by some molecules such as cell surface and extra cellular matrix Glycoconjugates terminal sugars. In this study we used lectin histochemichal technique to evaluate distribution of the Glycoconjugates and their changes during development of neurohypophysis. Materials and Methods: This experimental study carried on 40 female and 20 male adult Rats. After mating and appointment day zero of pregnancy, pregnant Rats were sacrificed from days 10-20 of gestation and their embryos were collected for histochemical study. The serial section of head specimens were fixed and incubated with different HRP-lectins from Orange fungus (OFA) Vicica villosa (VVA), Glycine max (SBA), Wistaria floribunda (WFA), peanut (PNA), Griffonia simplicfolia (GSA1-B4), Lotus tetragonolobus (LTA) and Ulex Europeus (UEA-1). OFA, LTA and UEA-1 lectins are specific for terminal sugars α-L–Fucose and WFA, SBA, VVA and PNA are specific for D-GalNAc, α, ß-D-GalNAc and GalNAc, D-Gal-(ß-1-3)- D-GalNAc of complex glycoconjugates respectively. Results: Our findings demonstrated that the reaction of neurohypophysis cells with OFA initiated from gestational GD10 and increased to GD15 (P |
| Databáze: | Directory of Open Access Journals |
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