Silk Fibroin Nanoparticles Functionalized with Fibronectin for Release of Vascular Endothelial Growth Factor to Enhance Angiogenesis
| Autor: | Payam Zahedi, Negar Hassani Besheli, Mehdi Farokhi, Fatemeh Mottaghitalab, Amin Sohrabi, Sohrab Ali Ghorbanian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Journal of Natural Fibers, Vol 19, Iss 14, Pp 9223-9234 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1544-0478 1544-046X 15440478 |
| DOI: | 10.1080/15440478.2021.1982814 |
| Popis: | This work aimed at enhancing the angiogenesis in the presence of silk fibroin nanoparticles functionalized with fibronectin (FN-SF NPs) for controlled release of vascular endothelial growth factor (VEGF). The FN-SF NP samples were synthesized via the desolvation method and then VEGF was encapsulated into them. The physicochemical properties of the VEGF-loaded FN-SF NPs were evaluated by field-emission scanning electron microscopy (FE-SEM), dynamic light scattering (DLS), and Fourier transform infrared spectroscopy (FTIR). Results showed the spherical morphology, size of ~90 nm with a polydispersity index of 0.21, and the presence of a characteristic peak in 1063 cm−1 owing to functionalizing between SF NPs and FN. Afterward, the VEGF release from the SF NPs with and without FN functionalization was carried out utilizing enzyme-linked immunosorbent assay (ELISA) and the outcomes revealed that the FN-SF NPs have slower VEGF release than that of unfunctionalized sample with difference of approximately 10%. Eventually, the bioactivity of VEGF released from SF NPs, and FN-SF NPs was assessed using human umbilical vein endothelial cells (HUVECs) culture and the results exhibited a remarkable increase of their proliferation on FN-SF NPs owing to the presence of VEGF even after 10 days. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|