| Autor: |
Rob G.J.A. Zuiker, Catherine Tribouley, Zuzana Diamant, J. Diderik Boot, Adam F. Cohen, K. Van Dyck, I. De Lepeleire, Veronica M. Rivas, Vladislav A. Malkov, Jacobus Burggraaf, Marcella K. Ruddy |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
European Clinical Respiratory Journal, Vol 3, Iss 0, Pp 1-17 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2001-8525 |
| DOI: |
10.3402/ecrj.v3.31324 |
| Popis: |
Background: Inhaled allergen challenge is a validated disease model of allergic asthma offering useful pharmacodynamic assessment of pharmacotherapeutic effects in a limited number of subjects. Objectives: To evaluate whether an RNA signature can be identified from induced sputum following an inhaled allergen challenge, whether a RNA signature could be modulated by limited doses of inhaled fluticasone, and whether these gene expression profiles would correlate with the clinical endpoints measured in this study. Methods: Thirteen non-smoking, allergic subjects with mild-to-moderate asthma participated in a randomised, placebo-controlled, 2-period cross-over study following a single-blind placebo run-in period. Each period consisted of three consecutive days, separated by a wash-out period of at least 3 weeks. Subjects randomly received inhaled fluticasone ((FP) MDI; 500 mcg BID×5 doses in total) or placebo. On day 2, house dust mite extract was inhaled and airway response was measured by FEV1 at predefined time points until 7 h post-allergen. Sputum was induced by NaCl 4.5%, processed and analysed at 24 h pre-allergen and 7 and 24 h post-allergen. RNA was isolated from eligible sputum cell pellets ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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