piR-1919609 Is an Ideal Potential Target for Reversing Platinum Resistance in Ovarian Cancer
| Autor: | Ying Yan MD, Dan Tian MD, Bingbing Zhao PhD, Zhuang Li PhD, Zhijiong Huang PhD, Kuina Li MD, Xiaoqi Chen MD, Lu Zhou MD, Yanying Feng MD, Zhijun Yang PhD |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Technology in Cancer Research & Treatment, Vol 23 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1533-0338 15330338 |
| DOI: | 10.1177/15330338241249692 |
| Popis: | Purpose PIWI-interacting RNAs (piRNAs) are a type of noncoding small RNA that can interact with PIWI-like RNA-mediated gene silencing (PIWIL) proteins to affect biological processes such as transposon silencing through epigenetic effects. Recent studies have found that piRNAs are widely dysregulated in tumors and associated with tumor progression and a poor prognosis. Therefore, this study aimed to investigate the effect of piR-1919609 on the proliferation, apoptosis, and drug resistance of ovarian cancer cells. Methods In this study, we used small RNA sequencing to screen and identify differentially expressed piRNAs in primary ovarian cancer, recurrent ovarian cancer, and normal ovaries. A large-scale verification study was performed to verify the expression of piR-1919609 in different types of ovarian tissue, including ovarian cancer tissue and normal ovaries, by RT–PCR and to analyze its association with the clinical prognosis of ovarian cancer. The expression of PIWILs in ovarian cancer was verified by RT–PCR, Western blotting and immunofluorescence. The effects of piR-1919609 on ovarian cancer cell proliferation, apoptosis and drug resistance were studied through in vitro and in vivo models. Results (1) piR-1919609 was highly expressed in platinum-resistant ovarian cancer tissues (p |
| Databáze: | Directory of Open Access Journals |
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