The growth and production of antimicrobial compounds from Lactobacillus plantarum IIA-1A5 on cheese whey medium
| Autor: | A. Mutmainna, I. I. Arief, C. Budiman |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of the Indonesian Tropical Animal Agriculture, Vol 46, Iss 2, Pp 173-184 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2087-8273 2460-6278 |
| DOI: | 10.14710/jitaa.46.2.173-184 |
| Popis: | Plantaricin IIA-1A5 is a bacteriocin produced by Lactobacillus plantarum IIA-1A5 which is isolated from Indonesian beef, and it inhibits activity of Gram negative and positive pathogenic bacteria. However, preparation of the antibacterial agent for further applications or studies is costly due to the usage of a bacterial medium. Therefore, this study was aimed to investigate the feasibility of cheese whey as a growth medium for production of the bacteriocin. The growth curve of Lactobacillus plantarum IIA-1A5 in producing antimicrobial compounds was found to occur in the logarithmic phase with an incubation time of 28 and 32 hours. Purification of plantaricin IIA-1A5 produced peptides with a molecular weight of 9.59 kDa consisting of whey and whey+ (20g/L sucrose, 12.5 g/L tryptone and 7.5 g/L yeast extract); thus, the peptide was grouped as class IIa ( |
| Databáze: | Directory of Open Access Journals |
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