| Autor: |
Yan-yan SHAO, Lin FU, Meng-ting ZHU, Ying NAN, Heng YANG, Zong-sheng ZHAO |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Kafkas Universitesi Veteriner Fakültesi Dergisi, Vol 27, Iss 6, Pp 681-690 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1309-2251 |
| DOI: |
10.9775/kvfd.2021.26080 |
| Popis: |
Given few reports on the interaction of miRNA-665/target gene pair, we aimed to construct a dual luciferase reporter gene vector for 3"-untranslated region (3"-UTR) of the haematopoietic prostaglandin D synthase (HPGDS) gene and elucidate the underlying molecular mechanism of miR-665 regulation of HPGDS. Bioinformatics software was used to predict miR-665 targeting of 3"-UTR region of HPGDS gene. The reliability of the synthetic psiCHECK-HPGDS-w/m-3"-UTR was determined using double luciferase digestion method. Then, miR-665 mimic/negative control was separately co-transfected with sheep luteal cells, and then, luciferase activity and HPGDS expression were detected. Results showed that 3"-UTR wild-type (psiCHECK2-HPGDS-w-3"UTR) and mutant (psiCHECK2-HPGDS-m-3"UTR) expression vectors for HPGDS were successfully constructed, and dual luciferase reporter gene assay showed that the expression of relative luciferase activity was inhibited in the w-3"-UTR group, with 52% decrease compared to the blank/negative control group, and the difference was statistically significant (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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