ASD-GFP Vectors for In Vivo Expression Technology in Pseudomonas aeruginosa and Other Gram-Negative Bacteria
| Autor: | M. Handfield, H.P. Schweizer, M.J. Mahan, F. Sanschagrin, T. Hoang, R.C. Levesque |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 24, Iss 2, Pp 261-264 (1998) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/98242st02 |
| Popis: | We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate β-semialdehyde dehydrogenase (asd) gene as a selectable marker and β-galactosidase (pIVPRO, 10.88 kb) or mutant green fluorescent protein with enhanced fluorescence properties (mut3GFP, pIVET-GFP, 5.48 kb) as reporter gene systems. The proposed strategies can be adapted for use in most Gram-negative bacteria. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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