Effects of Capsaicin on Adipogenic Differentiation in Bovine Bone Marrow Mesenchymal Stem Cell

Autor: Jin Young Jeong, Sekar Suresh, Mi Na Park, Mi Jang, Sungkwon Park, Kuppannan Gobianand, Seungkwon You, Sung-Heom Yeon, Hyun-Jeong Lee
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Asian-Australasian Journal of Animal Sciences, Vol 27, Iss 12, Pp 1783-1793 (2014)
Druh dokumentu: article
ISSN: 1011-2367
1976-5517
DOI: 10.5713/ajas.2014.14720
Popis: Capsaicin is a major constituent of hot chili peppers that influences lipid metabolism in animals. In this study, we explored the effects of capsaicin on adipogenic differentiation of bovine bone marrow mesenchymal stem cells (BMSCs) in a dose- and time-dependent manner. The BMSCs were treated with various concentrations of capsaicin (0, 0.1, 1, 5, and 10 μM) for 2, 4, and 6 days. Capsaicin suppressed fat deposition significantly during adipogenic differentiation. Peroxisome proliferator-activated receptor gamma, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer binding protein alpha, fatty acid binding protein 4, and stearoyl-CoA desaturase expression decreased after capsaicin treatment. We showed that the number of apoptotic cells increased in dose- and time-dependent manners. Furthermore, we found that capsaicin increased the expression levels of apoptotic genes, such as B-cell lymphoma 2-associated X protein and caspase 3. Overall, capsaicin inhibits fat deposition by triggering apoptosis.
Databáze: Directory of Open Access Journals