Construction and Evaluation of the Brucella Double Gene Knock-out Vaccine Strain MB6 Δbp26ΔwboA (RM6)
| Autor: | Bochang Shi, Xinyu Li, Bo Li, Naxin Zheng, Min Li, Yuan Liu, Chunhe Li, Fang Yan, Wei He, Liyan Zhao, Huabin Li, Chongyu Tian, Yuanqiang Zheng, Zhongpeng Zhao |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Zoonoses, Vol 2, Iss 1, p 971 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2737-7474 2737-7466 |
| DOI: | 10.15212/ZOONOSES-2022-0031 |
| Popis: | Brucellosis is a serious zoonotic infection worldwide. To date, vaccination is the most effective measure against brucellosis. This study was aimed at obtaining a vaccine strain that has high protective efficacy and low toxicity, and allows vaccination to be differentiated from infection. Using homologous recombination, we constructed a double gene-deletion Brucella strain MB6 Δbp26ΔwboA (RM6) and evaluated its characteristics, safety and efficacy. The RM6 strain had good proliferative ability and stable biological characteristics in vivo and in vitro. Moreover, it had a favorable safety profile and elicited specific immune responses in mice and sheep. The RM6 strain may have substantial practical application value. |
| Databáze: | Directory of Open Access Journals |
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