| Autor: |
Vanihamín Domínguez, Itzen Aguiñiga, Leticia Moreno, Beatriz Torres, Edelmiro Santiago-Osorio |
| Jazyk: |
English<br />Spanish; Castilian |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 4, Pp 571-576 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
0120-4157 |
| DOI: |
10.7705/biomedica.v37i4.3604 |
| Popis: |
Introduction: Sodium caseinate, a casein salt, is a proinflammatory agent in mice, and it is able to induce granulopoiesis in vivo and to increase the production of cytokines, which is key for this biological process. Objective: To assess whether sodium caseinate is able to induce a biological effect on cells from lymphoid origin and the production of cytokines involved in this lineage in vivo. Materials and methods: We used female BALB /c mice from 8 to 12 weeks old. The animals were injected intraperitoneally (IP) with 1 ml of sodium caseinate (10% PBS w/v) four times every 48 hours. The B cell populations and the incorporation of BrdU were analyzed by flow cytometry. Detection of interleukin-7 was assessed by ELISA (Enzyme-Linked ImmunoSorbent Assay). Results: We established that after intraperitoneal injection, the number of B lymphocytes 220+ from the spleen of mice treated with sodium caseinate increased compared to those that only received the vehicle (89.01±1.03 vs 75.66 ± 2.08), and the same was observed with the incorporation of BrdU in B220 + cells (38.59±4.48 vs 11.82±1.04 respectively). We also established that the concentration of interleukin-7 (IL-7) in the serum of mice treated with sodium caseinate increased compared to those that only received the vehicle (62.1 ± 17.5 vs 26.9 ± 4.4 pg/ml). Conclusion: Sodium caseinate was able to increase the number of B lymphocytes in the spleen; it also induced IL-7 production, a cytokine that is key for the B cell lymphopoiesis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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